FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cul3, ScsβA
FlyBase ID
FBig0000104258
Interaction Network
Interactions Browser links
Cul3 network
ScsβA network
Reported Interactions
FBrf0231856-1.Y2H
Reference
Aram et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cul3 

Cul3T

bait

fused to GAL4 DNA-binding domain

Cul3 
Cul3 
ScsβA 

A-Sβ

prey

fused to GAL4 activation domain

ScsβA 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cul3 
TeNC domain
sufficient binding region
aa 1-181

coordinates relative to Cul3-PF

ScsβA 
Cul3-Klhl10 interacting region
sufficient binding region
aa 306-452

coordinates relative to skap-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Aram et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Aram et al., 2016)
FBrf0231856-4.coIP.WB
Reference
Aram et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cul3 

Cul3T

bait

FLAG tag

ScsβA 

A-Sβ

prey

HA tag

ScsβA 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ScsβA 
Cul3-Klhl10 interacting region
sufficient binding region
aa 306-452

coordinates relative to skap-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aram et al., 2016)
FBrf0231856-6.coIP.WB
Reference
Aram et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ScsβA 

A-Sβ

bait

HA tag

ScsβA 
Cul3 

Cul3T

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ScsβA 
Cul3-Klhl10 interacting region
sufficient binding region
aa 306-452

coordinates relative to skap-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aram et al., 2016)

Binding of Cul3 to skap is much stronger when klhl10 is cotransfected.

(Aram et al., 2016)
FBrf0231856-8.coIP.WB
Reference
Aram et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cul3 

Cul3T

bait

FLAG tag

ScsβA 

A-Sβ

prey

HA tag

ScsβA 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ScsβA 
Cul3-Klhl10 interacting region
sufficient binding region
aa 306-452

coordinates relative to skap-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aram et al., 2016)

Binding of skap to Cul3 is stronger upon cotransfection of klhl10.

(Aram et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)