FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
14-3-3ε, Rheb
FlyBase ID
FBig0000104265
Interaction Network
Interactions Browser links
14-3-3ε network
Rheb network
Reported Interactions
FBrf0232252-3.coIP.WB
Reference
Le et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait

FLAG tag

Rheb 

Rheb

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Le et al., 2016)
FBrf0232252-7.PD.WB
Reference
Le et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait

GST tag

Rheb 

Rheb

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Le et al., 2016)
FBrf0233454-907.coIP.MS
Reference
Vinayagam et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Rheb 

Rheb

bait

TAP tag

14-3-3ε 

14-3-3epsilon

prey

SAINT score = 0.9993

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Vinayagam et al., 2016)

Observed in one of three conditions tested: 30 minutes after insulin stimulation.

(Vinayagam et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)