FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
piwi, Su(z)12
FlyBase ID
FBig0000104473
Interaction Network
Interactions Browser links
piwi network
Su(z)12 network
Reported Interactions
FBrf0231095-10.coIP.WB
Reference
Peng et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Su(z)12 

Su(z)12

bait

FLAG tag

Su(z)12 
piwi 

Piwi

prey

Myc tag

piwi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
piwi 
interaction domain
sufficient binding region
aa 160-257

construct "d"

Su(z)12 
interaction domain
sufficient binding region
aa 380-500

construct "VI", coordinates relative to Su(z)12-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Peng et al., 2016)
FBrf0231095-7.coIP.WB
Reference
Peng et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait

FLAG tag

Su(z)12 

Su(z)12

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Peng et al., 2016)

piwi coimmunoprecipitates Su(z)12 and esc, but not E(z) or corto in this co-transfection/coimmunoprecipitation assay. Association of piwi with PRC2 complex is thus mediated by Su(z)12 and esc. Note that piwi does not modulate in vitro histone methylation activity of reconstituted PRC2.

(Peng et al., 2016)
FBrf0231095-9.coIP.WB
Reference
Peng et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait

Myc tag

piwi 
Su(z)12 

Su(z)12

prey

FLAG tag

Su(z)12 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
piwi 
interaction domain
sufficient binding region
aa 160-257

construct "d"

Su(z)12 
interaction domain
sufficient binding region
aa 380-500

construct "VI", coordinates relative to Su(z)12-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Peng et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)