FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
yki, Mad
FlyBase ID
FBig0000104476
Interaction Network
Interactions Browser links
yki network
Mad network
Reported Interactions
FBrf0212763-1.coIP.WB
Reference
Oh and Irvine, 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

V5 tag

yki 
Mad 

Mad

prey

FLAG tag

Mad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW domains
necessary binding region
Mad 
PPXY motif
mutation decreasing interaction

Y225A, coordinates relative to Mad-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Oh and Irvine, 2011)
FBrf0227583-1.coIP.WB
Reference
Alarcón et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

FLAG tag

Mad 
yki 

Yorkie

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mad 
linker phosphorylation sites S202-S212
mutation disrupting interaction
aa 202-212

S202A, S212A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Alarcón et al., 2009)
FBrf0227583-2.coIP.WB
Reference
Alarcón et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yorkie

bait

HA tag

yki 
Mad 

Mad

prey

FLAG tag

Mad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mad 
linker phosphorylation sites S202-S212
mutation disrupting interaction
aa 202-212

S202A, S212A

yki 
WW domains
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Alarcón et al., 2009)
FBrf0247927-8.coIP.WB
Reference
Srivastava et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Firefly Luciferase tag

Mad 

MAD

prey

Renilla Luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Srivastava et al., 2021)

No difference in yki-cbt interaction with different yki isoforms including or excluding exon 3.

(Srivastava et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)