FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
chb, msps
FlyBase ID
FBig0000104605
Interaction Network
Interactions Browser links
chb network
msps network
Reported Interactions
FBrf0229697-1.coIP.WB
Reference
Trogden and Rogers, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
msps 

Msps

bait
chb 

Orbit

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Trogden and Rogers, 2015)
FBrf0229697-2.coIP.WB
Reference
Trogden and Rogers, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
chb 

Orbit

bait

GFP tag

chb 
msps 

Msps

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
chb 
interaction domain 1
mutation increasing interaction

Mutation of two phosphorylation sites.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction requires the mutation of two phosphorylation sites of chb.

(Trogden and Rogers, 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Trogden and Rogers, 2015)
FBrf0229697-3.coIP.WB
Reference
Trogden and Rogers, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
chb 

Orbit

bait

GFP tag

chb 
msps 

Msps

prey

mCherry tag

msps 
msps 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
chb 
interaction domain 1
sufficient binding region
aa 1071-1492
msps 
interaction domain 1
sufficient binding region
aa 1406-1596
msps 
interaction domain 2
mutation disrupting interaction

EKDWVR mutated to KKKAAA (3K3A)

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Trogden and Rogers, 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)