FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-8, ush
FlyBase ID
FBig0000104618
Interaction Network
Interactions Browser links
mir-8 network
ush network
Reported Interactions
FBrf0209508-1.miR-mRNA.LUC
Reference
Hyun et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mir-8 

miR-8

bait
ush 

ush

prey
ush 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ush 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

luciferase reporter assay

(Hyun et al., 2009)

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Hyun et al., 2009)
FBrf0209508-8.miR-mRNA.PCR
Reference
Hyun et al., 2009
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval fat body / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-8 

miR-8

bait
ush 

ush

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mutation of mir-8 increases ush mRNA levels.

(Hyun et al., 2009)

Source was larval fat body of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Hyun et al., 2009)
FBrf0209508-9.miR-mRNA.WB
Reference
Hyun et al., 2009
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval fat body / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-8 

miR-8

bait
ush 

ush

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mutation of mir-8 increases ush protein levels.

(Hyun et al., 2009)

Source was larval fat body of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Hyun et al., 2009)
FBrf0225101-1.miR-mRNA.LUC
Reference
Lee and Hyun, 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-8 

mir-8

bait
ush 

Ush

prey
ush 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ush 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Lee and Hyun, 2014)

Luciferase reporter assay.

(Lee and Hyun, 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)