Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.