FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
wg, Cow
FlyBase ID
FBig0000105035
Interaction Network
Interactions Browser links
wg network
Cow network
Reported Interactions
FBrf0226649-1.coIP.WB
Reference
Chang and Sun, 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wg 

Wg

bait

GFP tag

Cow 

Cow

prey

FLAG tag

Cow 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Chang and Sun, 2014)
FBrf0226649-2.coIP.WB
Reference
Chang and Sun, 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cow 

Cow

bait

FLAG tag

wg 

Wg

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Chang and Sun, 2014)
FBrf0226649-3.coIP.WB
Reference
Chang and Sun, 2014
Description
physical association
Assay
Collection
Source/Stage
organism / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cow 

Cow

bait
wg 

Wg

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Chang and Sun, 2014)
FBrf0226649-4.coIP.WB
Reference
Chang and Sun, 2014
Description
physical association
Assay
Collection
Source/Stage
organism / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wg 

Wg

bait

HA tag

Cow 

Cow

prey
Cow 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic constructendogenous gene; prey produced from .

(Chang and Sun, 2014)
FBrf0226649-5.coIP.WB
Reference
Chang and Sun, 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
wg 

Wg

bait

HA tag

Cow 

Cow

prey

FLAG tag

Cow 
Cow 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cow 
glycosaminoglycan addition sites 1 and 2
mutation disrupting interaction
aa 287-332

S287A and G288A, S331A and G332A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was culture supernatant of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chang and Sun, 2014)

Constructs were transfected separately and then mixed for immunoprecipitation tests.

(Chang and Sun, 2014)
FBrf0226649-6.coIP.WB
Reference
Chang and Sun, 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cow 

Cow

bait

FLAG tag

Cow 
wg 

Wg

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cow 
glycosaminoglycan addition sites 1 and 2
mutation disrupting interaction
aa 287-332

S287A and G288A, S331A and G332A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was culture supernatant of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chang and Sun, 2014)

Constructs were transfected separately and then mixed for immunoprecipitation tests.

(Chang and Sun, 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)