FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
BEAF-32, Chro
FlyBase ID
FBig0000105037
Interaction Network
Interactions Browser links
BEAF-32 network
Chro network
Reported Interactions
FBrf0226086-1.coIP.WB
Reference
Vogelmann et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chro 

Chromator

bait

His tag

Chro 
BEAF-32 

BEAF32

prey

MBP tag, His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Chro 
C-terminal region
sufficient binding region
aa 601-926
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Vogelmann et al., 2014)
FBrf0226086-5.coIP.WB
Reference
Vogelmann et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Chro 

Chromator

bait
BEAF-32 

BEAF32

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vogelmann et al., 2014)
FBrf0226086-9.EMSA.A
Reference
Vogelmann et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BEAF-32 

BEAF32

bait

MBP tag, His tag

Chro 

Chromator

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

BEAF-32, Cp190 and Chro were incubated, alone or in combination, with genomic DNA spanning an insulator at the Tudor-SN locus.

(Vogelmann et al., 2014)

Chro has no observed DNA binding activity on its own, but it supershifts the BEAF-32 protein-DNA complex.

(Vogelmann et al., 2014)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion pr

(Vogelmann et al., 2014)
FBrf0249398-1.coIP.WB
Reference
Melnikova et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
BEAF-32 

BEAF-32

bait
Chro 

Chriz

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Melnikova et al., 2021)
FBrf0249398-4.Y2H
Reference
Melnikova et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BEAF-32 

BEAF-32

bait

fused to GAL4 DNA-binding domain

BEAF-32 
BEAF-32 
BEAF-32 
BEAF-32 
BEAF-32 
Chro 

Chriz

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
BEAF-32 
leucine zipper and BESS domains; residues 200-283
mutation disrupting interaction
aa 200-283

coordinates relative to BEAF-32-PA

BEAF-32 
BESS domain residues 230-283
mutation decreasing interaction
aa 1-200

coordinates relative to BEAF-32-PA

BEAF-32 
BED and BESS domains residues 1-58 and 230-283
mutation disrupting interaction
aa 1-58; 230-283

coordinates relative to BEAF-32-PA

BEAF-32 
BED and LZ domains residues 1-230
mutation disrupting interaction
aa 1-230

coordinates relative to BEAF-32-PA

BEAF-32 
BED domain residues 1-200
mutation decreasing interaction
aa 1-200

coordinates relative to BEAF-32-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Melnikova et al., 2021)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2021)
FBrf0249398-6.Y2H
Reference
Melnikova et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chro 

Chriz

bait

fused to GAL4 DNA-binding domain

Chro 
Chro 
Chro 
BEAF-32 

BEAF-32

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Chro 
positively charged domain residues 273-621
sufficient binding region
aa 273-621

coordinates relative to Chro-PA

Chro 
interaction domain residues 273-503
mutation disrupting interaction
aa 273-503

coordinates relative to Chro-PA

Chro 
interaction domain residues 1-273 and 503-926
mutation decreasing interaction
aa 1-273;503-926

coordinates relative to Chro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Melnikova et al., 2021)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2021)
FBrf0249398-7.PD.WB
Reference
Melnikova et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chro 

Chriz

bait

GST tag

BEAF-32 

BEAF-32

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein.

(Melnikova et al., 2021)
FBrf0253430-27.PD.MS
Reference
Kaushal et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chro 

Chro

bait

GFP tag

Chro 
BEAF-32 

BEAF-32

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Chro 
C terminal domain
sufficient binding region
aa 613-926

coordinates relative to Chro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.

(Kaushal et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)