FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000105224
Interaction Network
Interactions Browser links
gce network
ftz-f1 network
Reported Interactions
FBrf0215576-2.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gce 

GCE

bait

GST tag

FTZ-F1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction between ftz-f1 and gce was enhanced following treatment of S2 cells with methoprene.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0217595-2.2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gce 

gce

bait

fused to GAL4 DNA-binding domain

gce 

ftz-f1

prey

fused to p65 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal domain
mutation decreasing interaction
aa 789-803

coordinates relative to ftz-f1-PA

gce 
interaction domain residue 418
mutation decreasing interaction
aa 418

L418A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

FBrf0247134-1.NMR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gce 

GCE

neutral component
gce 
gce 

GCE

neutral component
gce 
gce 

GCE

neutral component

FTZ-F1

neutral component

FTZ-F1

neutral component

FTZ-F1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gce 
C-terminal domain
sufficient binding region
aa 661-959

coordinates relative to gce-PC

LBD domain
sufficient binding region
aa 782-1027

coordinates relative to ftz-f1-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

FBrf0247134-2.NMR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gce 

GCE

neutral component

peptide

gce 

GCE

neutral component

peptide

gce 
gce 

GCE

neutral component

peptide

gce 

FTZ-F1

neutral component

FTZ-F1

neutral component

FTZ-F1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gce 
NR-box
sufficient binding region
aa 686-694

coordinates relative to gce-PC

LBD domain
sufficient binding region
aa 782-1027

coordinates relative to ftz-f1-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; peptide synthesized in vitro.

FBrf0247134-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gce 

GCE

bait

FLAG tag

gce 

FTZ-F1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gce 
C-terminal domain
sufficient binding region
aa 661-959

coordinates relative to gce-PC

LBD domain
sufficient binding region
aa 782-1027

coordinates relative to ftz-f1-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0257441-3.NMR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gce 

Gce

neutral component
gce 
gce 

Gce

neutral component
gce 
gce 

Gce

neutral component

Ftz-F1

neutral component

His tag

Ftz-F1

neutral component

His tag

Ftz-F1

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gce 
NR box
sufficient binding region
aa LEKAVLRLIQNLQKSGENG

coordinates relative to Met-PA

Ligand binding domain
sufficient binding region
aa 786-1027
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; gce peptide chemically synthesized; ftz-f1 produced as a recombinant fusion protein in bacterial system.

FBrf0257441-6.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gce 

Gce

bait

FLAG-YFP tag

Ftz-F1

prey

CFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ligand binding domain
sufficient binding region
aa 786-1027
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)