FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sas-4, βTub56D
FlyBase ID
FBig0000105227
Interaction Network
Interactions Browser links
Sas-4 network
βTub56D network
Reported Interactions
FBrf0216370-12.coIP.WB
Reference
Gopalakrishnan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

TAP tag

βTub56D 

β-Tubulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Gopalakrishnan et al., 2011)

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

(Gopalakrishnan et al., 2011)
FBrf0216370-32.coIP.WB
Reference
Gopalakrishnan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

GFP tag

Sas-4 
Sas-4 
Sas-4 
βTub56D 

β-Tubulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sas-4 
tubulin binding domain
sufficient binding region
aa 1-190
Sas-4 
tubulin binding domain
necessary binding region
aa 1-150
Sas-4 
PN2-3 domain
necessary binding region
aa 103-121
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Gopalakrishnan et al., 2011)
FBrf0216370-37.PD.WB
Reference
Gopalakrishnan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

GST tag

Sas-4 
Sas-4 
βTub56D 

β-Tubulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sas-4 
tubulin binding domain
sufficient binding region
aa 1-190
Sas-4 
PN2-3 domain
necessary binding region
aa 103-121
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Gopalakrishnan et al., 2011)
FBrf0219801-42.PD.WB
Reference
Gopalakrishnan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

GST tag

Sas-4 
Sas-4 
βTub56D 

β-tubulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sas-4 
N-terminal region
sufficient binding region
aa 1-190
Sas-4 
tubulin binding domain
mutation disrupting interaction
aa 114-115

R114E, R115E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Gopalakrishnan et al., 2012)
FBrf0219801-51.coIP.WB
Reference
Gopalakrishnan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait
βTub56D 

Tubulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gopalakrishnan et al., 2012)

The interaction of Sas-4 with βTub56D is disrupted by the addition of a non-hydrolyzable GTP analog, GMPCPP, to the extract.

(Gopalakrishnan et al., 2012)

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

(Gopalakrishnan et al., 2012)
FBrf0219801-8.coIP.WB
Reference
Gopalakrishnan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait
βTub56D 

Tubulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gopalakrishnan et al., 2012)

Tubulin coimmunoprecipitates with Sas-4 and co-fractionates with Sas-4 across the entire sucrose gradient.

(Gopalakrishnan et al., 2012)

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

(Gopalakrishnan et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)