FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Klp64D, arm
FlyBase ID
FBig0000105262
Interaction Network
Interactions Browser links
Klp64D network
arm network
Reported Interactions
FBrf0225822-1.coIP.WB
Reference
Vuong et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

Klp64D

bait

FLAG tag

arm 

Arm

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

arm coimmunoprecipitates with Klp64D, but not with Klp61F.

(Vuong et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Vuong et al., 2014)
FBrf0225822-2.coIP.WB
Reference
Vuong et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

Klp64D

bait
arm 

Arm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vuong et al., 2014)
FBrf0225822-3.coIP.WB
Reference
Vuong et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait
Klp64D 

Klp64D

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vuong et al., 2014)
FBrf0225822-4.PD.WB
Reference
Vuong et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

Klp64D

bait

GST tag

Klp64D 
arm 

Arm

prey

MBP tag

arm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
C-terminal tail domain
sufficient binding region
aa 582-677
arm 
Arm repeats region
sufficient binding region
aa 141-670

coordinates relative to arm-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Vuong et al., 2014)
FBrf0246513-1.PD.WB
Reference
Vuong et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

Klp64D

bait

GST tag

Klp64D 
Klp64D 
arm 

arm

prey

MBP tag

arm 
arm 
arm 
arm 
arm 
arm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
AR2 domain
sufficient binding region
aa 189-231

coordinates relative to arm-PA

arm 
AR2 domain
necessary binding region
aa 189-231

coordinates relative to arm-PA

arm 
AR3 domain
sufficient binding region
aa 232-272

coordinates relative to arm-PA

arm 
AR7 domain
sufficient binding region
aa 399-437

coordinates relative to arm-PA

arm 
AR8 domain
sufficient binding region
aa 438-481

coordinates relative to arm-PA

arm 
AR10 domain
sufficient binding region
aa 528-595

coordinates relative to arm-PA

Klp64D 
tail domain
sufficient binding region
Klp64D 
tail domain
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Vuong et al., 2020)
FBrf0246513-2.coIP.WB
Reference
Vuong et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

Klp64D

bait

FLAG tag

arm 

arm

prey

Myc tag

arm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
AR2 domain
sufficient binding region
aa 189-231

coordinates relative to arm-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Vuong et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)