Source was live S2 cells; proteins produced from transfected constructs.

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

Source was live S2 cells; bait produced from transfected construct; prey produced from transfected construct, secreted and collected in medium.

The ectodomain of ds fused to alkaline phosphatase is secreted and collected in the medium, which is then added to cells expressing ft on the cell surface. The degree of ds-ft interaction is inferred by quantitation of AP activity adhering to cells. Coexpression of kinase active fj with ft promotes the ds-ft interaction, while coexpression of kinase active fj with ds inhibits the ds-ft interaction.

Source was live S2 cells; bait produced from transfected construct; prey produced from transfected construct, secreted and collected in medium.

The ectodomain of ft fused to alkaline phosphatase is secreted and collected in the medium, which is then added to cells expressing ds on the cell surface. The degree of ds-ft interaction is inferred by quantitation of AP activity adhering to cells.

Interaction in vitro; bait produced from transfected construct, secreted and immunopurified; prey produced from transfected construct, secreted and collected in medium.

The cadherin 1-10 domain of ds fused to alkaline phosphatase is secreted and collected in the medium, which is then added to immunopurified cadherin 1-10 domain of ft in vitro. The degree of ds-ft interaction is inferred by quantitation of AP activity. Coexpression of fj with ft or treatment of purified ft with purified fj promotes the ds-ft interaction, while treatment of ft with phosphatase inhibits the ds-ft interaction.

Source was fixed S2 cells; proteins produced from transfected constructs.

Bait protein was fused to the ed cell adhesion protein, which targets the chimera to cell to cell contacts. The ability of this chimera to recruit prey protein to cell contact sites taken as evidence of direct physical interaction.