Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Three-hybrid system: yeast LexA-BD/MS2-SL/GAL4-AD. LexA-BD fused to phage MS2 coat protein, which binds MS2-SL.
Interaction in vitro; bait produced and labeled in vitro; prey produced as a recombinant fusion protein in bacterial system.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Three-hybrid system: yeast GAL4-BD/MS2-SL/GAL4-AD. GAL4-BD fused to pum.
Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system; RNA synthesized in vitro.
Source was cell extract of S2 cell line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Positive control.
