"ΔNt" construct, coordinates relative to imd-PA
"DD" construct, coordinates relative to imd-PA
Two-hybrid system: yeast LexA-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Anti-imd used for immunoprecipitation, with anti-Myc used to detect both bait and prey proteins.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
