FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Tl, Myd88
FlyBase ID
FBig0000107842
Interaction Network
Interactions Browser links
Tl network
Myd88 network
Reported Interactions
FBrf0139798-1.coIP.WB
Reference
Horng and Medzhitov, 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Tl 

Toll 10b

bait

constitutively active Tl[10b] form

Myd88 

DMyD88

prey

V5 tag

Myd88 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myd88 
C-terminal region
sufficient binding region
aa 176-537

coordinates relative to Myd88-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Horng and Medzhitov, 2001)
FBrf0141679-1.coIP.WB
Reference
Tauszig-Delamasure et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Tl 

Toll

bait

FLAG tag

Myd88 

DmMyD88

prey

V5 tag

Myd88 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myd88 
TIR domain
sufficient binding region
aa 224-376

coordinates relative to Myd88-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tauszig-Delamasure et al., 2002)

Myd88 binds Tl but not Tehao, Toll-6 or Tollo.

(Tauszig-Delamasure et al., 2002)
FBrf0167555-6.coIP.WB
Reference
Sun et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Myd88 

MyD88

bait

V5 tag

Tl 

Toll

prey

V5 tag

Tl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tl 
interaction domain
mutation increasing interaction
aa 781

C781Y

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)