Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced and labeled by in vitro transcription.
Biotin label was detected using HRP-linked reagent.
Biotin label was detected using HRP-linked reagent.
Interaction in vitro; bait produced as a tagged protein in S2-DRSC cell lysate; prey produced and labeled by in vitro transcription.
