FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association, colocalization
Interacting Genes
hid, rpr
FlyBase ID
FBig0000108553
Interaction Network
Interactions Browser links
hid network
rpr network
Reported Interactions
FBrf0211820-2.PD.A
Reference
Sandu et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rpr 

rpr

bait

GST tag

hid 

hid

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Sandu et al., 2010)
FBrf0211820-5.PD.MW
Reference
Sandu et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rpr 

rpr

bait

GST tag

hid 

hid

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Sandu et al., 2010)
FBrf0211820-6.PD.MW
Reference
Sandu et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hid 

hid

bait

GST tag

rpr 

rpr

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Sandu et al., 2010)
FBrf0211820-7.coIP.WB
Reference
Sandu et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hid 

hid

bait

FLAG tag

rpr 

rpr

prey

Myc tag

rpr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rpr 
putative helical domain
mutation decreasing interaction
aa 34, 35

F34AL35A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sandu et al., 2010)
FBrf0211820-8.CL.FM
Reference
Sandu et al., 2010
Description
colocalization
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
hid 

hid

bait

Myc tag

rpr 

rpr

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2R+ or BT549 (human) cell line; bait produced from tagged transfected construct; prey produced from transfected construct.

(Sandu et al., 2010)

hid-Myc localizes to mitochondria while rpr-HA is broadly distributed. Coexpression of hid-Myc and rpr-HA results in rpr-HA localization to mitochondria.

(Sandu et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)