FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ten-m, cher
FlyBase ID
FBig0000108587
Interaction Network
Interactions Browser links
Ten-m network
cher network
Reported Interactions
FBrf0214744-1.Y2H
Reference
Zheng et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ten-m 

Ten-m

bait

fused to GAL4 DNA-binding domain

Ten-m 
cher 

Filamin

prey

fused to GAL4 activation domain

cher 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ten-m 
intracellular domain
sufficient binding region
aa 910-1822

coordinates relative to Ten-m-PB

cher 
C-terminal region
sufficient binding region
aa 2031-2210

coordinates relative to cher-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Zheng et al., 2011)

7 cher clones recovered in the Ten-m screen.

(Zheng et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Zheng et al., 2011)
FBrf0214744-2.Y2H
Reference
Zheng et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ten-m 

Ten-m

bait

fused to GAL4 DNA-binding domain

Ten-m 
cher 

Filamin

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ten-m 
FID domain
sufficient binding region
aa 910-1172

"seg1" construct, coordinates relative to Ten-m-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zheng et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Zheng et al., 2011)
FBrf0214744-3.PD.A
Reference
Zheng et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cher 

Filamin

bait

GST tag

Ten-m 

Ten-m

prey

[35]S label

Ten-m 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ten-m 
FID domain
sufficient binding region
aa 910-1172

"seg1" construct, coordinates relative to Ten-m-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Zheng et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)