FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO1, mir-317
FlyBase ID
FBig0000108938
Interaction Network
Interactions Browser links
AGO1 network
mir-317 network
Reported Interactions
FBrf0223644-9.coIP.NB
Reference
Abe et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait
mir-317 

mir-317

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult male flies; bait produced from endogenous gene; prey produced from endogenous gene.

(Abe et al., 2014)
FBrf0239572-5.coIP.NB
Reference
Lim et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait
mir-317 

miR-317-3p

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Binding shown for miR-317-3p isoform which corresponds to mir-317-RA. The AGO1-mir-317 interaction is decreased upon treatment of cells with ecdysone.

(Lim et al., 2018)

Source was S2 cell extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Lim et al., 2018)
FBrf0242088-4.coIP.A
Reference
Goh and Okamura, 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait
mir-317 

mir-317

prey

P[32] label

mir-317 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mir-317 
loop
sufficient binding region
nt 36-55

coordinates relative to mir-317-RM

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

AGO1 binds mir-317 less strongly than AGO2.

(Goh and Okamura, 2019)

Interaction in vitro; bait produced from S2R+ cell lysate; prey RNA oligo sythesized in vitro.

(Goh and Okamura, 2019)
FBrf0245520-8.coIP.NB
Reference
Lim et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / wandering third instar larval stagepharate adult stage P8
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait
mir-317 

miR-317-3p

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae and pupae of wild-type strain; bait produced from endogenous gene; prey produced from endogenous gene.

(Lim et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (4)