Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait purified from transfected S2 cell extract; prey purified from baculovirus-infected Sf9 cell extract.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Embryos were formaldehyde crosslinked before affinity purification.
