FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109094
Interaction Network
Interactions Browser links
brat network
hb network
Reported Interactions
FBrf0224581-1.EMSA.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brat 

brat

bait
brat 
brat 
brat 
hb 

hb

prey

[32]P label

hb 
hb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hb 
3-prime UTR
sufficient binding region
nt 2834-2935nt 2834-2860nt 2888-2914

binds at lower affinity than nt 2834-2935

binds at lower affinity than nt 2834-2935

hb 
boxA
mutation disrupting interaction
AAUCGUUGUCCUUUUGUUGUCGUUUUGUUGUCGUUUUGUUGUCGUAUUUUGUUGU

AAUCGUUGUCC to AAGCGGGGGCC

UUUUGUUGUCG to UUGGGGGGUCG

UUUUGUUGUCG to UUGGGUUGUCG

UUUUGUUGUCG to UUUUGGGGUCG

UAUUUUGUUGU to UAUUAAGAAGU

brat 
NHL domain
sufficient binding region
aa 756-1037

coordinates refer to brat-PA

brat 
NHL domain
mutation disrupting interaction
aa 875aa 847aa 802

R875A, coordinates refer to brat-PA

R847A, coordinates refer to brat-PA

H802L, coordinates refer to brat-PA

brat 
NHL domain
mutation decreasing interaction
aa 829

Y829A, coordinates refer to brat-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

Binding of brat to hb mRNA facilitated by prebinding mRNA with pum.

FBrf0224581-2.MST
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brat 

brat

bait

NT-647-NHS dye

brat 
hb 

hb

prey
hb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hb 
3-prime UTR
sufficient binding region
nt 2834-2935nt 2888-2914

binds at lower affinity than nt 2834-2935

brat 
NHL domain
sufficient binding region
aa 756-1037

coordinates refer to brat-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro transcription.

FBrf0224581-4.CH.SLS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brat 

brat

neutral component
brat 

brat

neutral component
brat 
brat 

brat

neutral component
brat 
hb 

hb

neutral component
hb 
hb 

hb

neutral component
hb 

hb

neutral component
hb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hb 
3-prime UTR
sufficient binding region
nt 2834-2935
brat 
NHL domain
sufficient binding region
aa 756-1037

coordinates refer to brat-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro transcription.

Binding stoichiometry 1:1 .

FBrf0224581-6.XL.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hb 

hb

bait
hb 
brat 

brat

prey
brat 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hb 
3-prime UTR
sufficient binding region
nt 2834-2935
brat 
NHL domain
sufficient binding region
aa 756-1037

coordinates refer to brat-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro transcription.

FBrf0242571-5.coIP.PCR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brat 

Brat

bait

Myc tag

hb 

Hb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

Source was cell extract of S2 cell line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0247403-4.coIP.PCR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brat 

Brat

bait

Myc tag

hb 

Hunchback

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)