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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109354
Interaction Network
Interactions Browser links
CG10939 network
Mer network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0226713-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CG10939

bait

GST tag

Mer 

Mer

prey

[35]S label

Mer 
Mer 
Mer 
Mer 
Mer 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain
sufficient binding region
aa 247-296
interaction domain
mutation decreasing interaction
aa 247-296
Mer 
FERM domain
sufficient binding region
aa 1-104aa 103-213

weak binding

weak binding

Mer 
interaction domain
sufficient binding region
aa 306-405
Mer 
FERM domain
necessary binding region
aa 306-355aa 330-380
Mer 
interaction domain residue 325
mutation decreasing interaction
aa 325

R325A

Mer 
interaction domain residue 335
mutation decreasing interaction
aa 335

R335L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0226713-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

Mer

bait

Myc tag

Mer 
Mer 
Mer 
Mer 

CG10939

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
interaction domain residue 325
mutation decreasing interaction
aa 325

R325A, interaction reduced 10% relative to WT.

Mer 
interaction domain residue 335
mutation decreasing interaction
aa 335

R335L, interaction reduced 30% relative to WT.

Mer 
interaction domain
mutation decreasing interaction
aa 325,335

R325A,R335L, interaction reduced 75% relative to WT.

Mer 
phosphorylation site residue 616
mutation decreasing interaction
aa 616

T616A, interaction reduced 90% relative to WT.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was pupae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0226713-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CG10939

bait

FLAG tag

Mer 

Mer

prey

HA tag

Mer 
Mer 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
interaction domain residue 335
mutation decreasing interaction
aa 335

R335L, interaction reduced 45% relative to WT.

Mer 
interaction domain
mutation decreasing interaction
aa 325,335

R325A,R335L, interaction reduced 70% relative to WT.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)