Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

A weak ttk-sinah interaction is detectable in the absence of phyl, but is stronger in the presence of phyl.

A C87A RING domain mutant of sinah was used to minimize degradation of ttk.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD