FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000110637
Interaction Network
Interactions Browser links
Arf1 network
Sec71 network
Reported Interactions
FBrf0235566-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arf1 

Arf79F

bait

GST tag

Arf1 

Sec71

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arf1 
GTP binding domain
mutation decreasing interaction
aa 71

Q71L, GTP locked form

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from extract of transfected S2 cells.

Interaction decreased with addition of non-hydrolyzable GTP analog.

FBrf0235566-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Sec71

bait

Myc tag

Arf1 

Arf79F

prey

FLAG tag

Arf1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arf1 
GTP binding domain
mutation decreasing interaction
aa 71

Q71L, GTP locked form

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0235566-3.ENZ.F
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Sec71

enzyme

GST tag

Sec71

enzyme

GST tag

Arf1 

Arf79F

enzyme target

His tag

Arf1 

Arf79F

enzyme target

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)