FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sqh, ci
FlyBase ID
FBig0000110668
Interaction Network
Interactions Browser links
sqh network
ci network
Reported Interactions
FBrf0233140-1.PD.MS
Reference
Liu et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
ci 

ci

bait

MBP tag

ci 
sqh 

sqh

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
Zn finger domains
sufficient binding region
aa 451-603
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from CME-W1-Cl.8+ cell extract.

(Liu et al., 2015)

Assay to identify proteins that interact with ci Zn fingers in Clone8 cells in absence of hh signaling.

(Liu et al., 2015)
FBrf0233140-2.coIP.WB
Reference
Liu et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
sqh 

sqh

bait

FLAG tag

ci 

ci

prey

Myc tag

ci 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
Zn finger domains
sufficient binding region
aa 451-603
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2015)

Full-length ci fails to interact with sqh.

(Liu et al., 2015)
FBrf0233140-3.coIP.WB
Reference
Liu et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc-unspecified
Participants
Corresponds to
Reported as
Role
Note
ci 

ci

bait

FLAG tag

ci 
sqh 

sqh

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
Zn finger domains
sufficient binding region
aa 451-603
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2015)

Full-length ci fails to interact with sqh.

(Liu et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)