FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO1, hpRNA:CR46342
FlyBase ID
FBig0000111073
Interaction Network
Interactions Browser links
AGO1 network
hpRNA:CR46342 network
Reported Interactions
FBrf0206362-33.coIP.PCR
Reference
Zhou et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

FLAG tag

hpRNA:CR46342 

esi-2.1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Zhou et al., 2008)
FBrf0210024-11a.coIP.NB
Reference
Miyoshi et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait
hpRNA:CR46342 

esi-sl-4

prey
hpRNA:CR46342 
hpRNA:CR46342 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpRNA:CR46342 
esi-sl-4
sufficient binding region
CUUCCGCUGGCUUUGAUUUUC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Miyoshi et al., 2010)
FBrf0212657-4.XL.A
Reference
Ameres et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hpRNA:CR46342 

esi-2.1

bait

[32]P label

AGO1 

Ago1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait synthesized and labeled in vitro; prey derived from wild-type embryo extract.

(Ameres et al., 2011)

siRNA esi-2.1 derived from hpRNA:CR46342 with a 5-prime U preferentially binds AGO1.

(Ameres et al., 2011)
FBrf0224072-6.coIP.NB
Reference
Yang et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

FLAG tag

hpRNA:CR46342 

esi-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Yang et al., 2014)
FBrf0249051-11.coIP.SEQ
Reference
Kingston and Bartel, 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago-1

bait

Flag-GST tag

hpRNA:CR46342 

CG4068

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kingston and Bartel, 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)