FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tsh, CtBP
FlyBase ID
FBig0000111962
Interaction Network
Interactions Browser links
tsh network
CtBP network
Reported Interactions
FBrf0216557-4.coIP.WB
Reference
Datta et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
tsh 

TSH

bait

Myc tag

CtBP 

CTBP

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Datta et al., 2011)
FBrf0263477-1.coIP.WB
Reference
Banerjee et al., 2025
Description
physical association
Assay
Collection
Source/Stage
eye-antennal disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tsh 

tsh

bait

EGFP tag

CtBP 

CtBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was third instar larval stage of eye-antennal discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Banerjee et al., 2025)
FBrf0263477-2.PD.WB
Reference
Banerjee et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CtBP 

CtBP

bait

GST tag

tsh 

tsh

prey

biotin label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Banerjee et al., 2025)
FBrf0263477-3.PD.WB
Reference
Banerjee et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tsh 

tsh

bait

GST tag

CtBP 

CtBP

prey

biotin label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Banerjee et al., 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)