FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
wash, CCDC53
FlyBase ID
FBig0000115854
Interaction Network
Interactions Browser links
wash network
CCDC53 network
Reported Interactions
FBrf0234908-4.TAP.MS
Reference
Jia et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
wash 

dWASH

bait

TAP tag

CCDC53 

dCCDC53

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Jia et al., 2010)
FBrf0246137-1.BN.WB
Reference
Verboon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
wash 

Wash

neutral component
wash 

Wash

neutral component
wash 

Wash

neutral component
CCDC53 

CCDC53

neutral component
CCDC53 

CCDC53

neutral component
CCDC53 

CCDC53

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc167 cell line; protein produced from endogenous genes.

(Verboon et al., 2020)

wash, CCDC53, Strump and FAM21 comigrate suggesting a complex; captured as a series of binary interactions.

(Verboon et al., 2020)
FBrf0246137-19.PD.A
Reference
Verboon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wash 

Wash

bait

GST tag

wash 
CCDC53 

CCDC53

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
wash 
interaction domain
necessary binding region
aa 61-64

coordinates relative to wash-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Verboon et al., 2020)
FBrf0246137-8.coIP.WB
Reference
Verboon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
wash 

Wash

bait
CCDC53 

CCDC53

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc167 cell line; protein produced from endogenous genes.

(Verboon et al., 2020)
FBrf0256485-14.PD.A
Reference
Davidson et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wash 

Wash

bait

GST tag

CCDC53 

CCDC53

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Davidson et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)