FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
krz, dx
FlyBase ID
FBig0000116128
Interaction Network
Interactions Browser links
krz network
dx network
Reported Interactions
FBrf0191265-1.Y2H
Reference
Mukherjee et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dx 

Dx

bait

fused to LexA DNA-binding domain

krz 

Krz

prey

fused to GAL4 activation domain

krz 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
krz 
N-terminal domain
sufficient binding region
aa 10-251
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Mukherjee et al., 2005)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Mukherjee et al., 2005)
FBrf0191265-2.TAP.MS
Reference
Mukherjee et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
dx 

Dx

bait

TAP tag

krz 

Krz

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Mukherjee et al., 2005)
FBrf0191265-3.coIP.WB
Reference
Mukherjee et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
krz 

Krz

bait

HA tag

dx 

Dx

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mukherjee et al., 2005)

Western blot probed with anti-Dx.

(Mukherjee et al., 2005)
FBrf0191265-4.coIP.WB
Reference
Mukherjee et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
dx 

Dx

bait

FLAG tag

krz 

Krz

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mukherjee et al., 2005)
FBrf0191265-5.coIP.WB
Reference
Mukherjee et al., 2005
Description
physical association
Assay
Collection
Source/Stage
imaginal disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
krz 

Krz

bait

HA tag

dx 

Dx

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Mukherjee et al., 2005)

Western blot probed with anti-Dx.

(Mukherjee et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)