FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rho1, Pkn
FlyBase ID
FBig0000117455
Interaction Network
Interactions Browser links
Rho1 network
Pkn network
Reported Interactions
FBrf0108231-1.PD.WB
Reference
Lu and Settleman, 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho1

bait

GST tag

Pkn 

Pkn

prey

FLAG tag

Pkn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pkn 
HR1 leucine zipper motif
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from transfected construct in BOSC cells.

(Lu and Settleman, 1999)
FBrf0201054-1.PD.WB
Reference
Betson and Settleman, 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho1

bait

GST tag

Pkn 

Pkn

prey

FLAG tag

Pkn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pkn 
ACC123 domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HEK293T cell extract.

(Betson and Settleman, 2007)
FBrf0244517-3.BiFC.LUC
Reference
Bagci et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho1

bait

Large BiT Luciferase fragment

Pkn 

Pkn

prey

Small BiT Luciferase fragment

Pkn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pkn 
Rho-binding domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2R+ cells; proteins produced from transfected constructs.

(Bagci et al., 2020)

Positive control

(Bagci et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (3)