FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CtBP, ush
FlyBase ID
FBig0000117488
Interaction Network
Interactions Browser links
CtBP network
ush network
Reported Interactions
FBrf0207210-1.coIP.WB
Reference
Biryukova and Heitzler, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ush 

Ush

bait

FLAG tag

CtBP 

dCtBP

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Biryukova and Heitzler, 2008)
FBrf0207210-2.PD.A
Reference
Biryukova and Heitzler, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CtBP 

dCtBP

bait

GST tag

ush 

Ush

prey

[35]S label

ush 
ush 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ush 
interaction domain
sufficient binding region
aa 348-677
ush 
interaction domain
mutation disrupting interaction
PLDLS

PLDLS to AAAAA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Biryukova and Heitzler, 2008)
External Crossreferences and Linkouts ( 0 )
References (1)