FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
e(y)2, su(Hw)
FlyBase ID
FBig0000119896
Interaction Network
Interactions Browser links
e(y)2 network
su(Hw) network
Reported Interactions
FBrf0201920-1.Y2H
Reference
Kurshakova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

E(y)2

bait

fused to LexA DNA-binding domain

su(Hw) 

Su(Hw)

prey

fused to GAL4 activation domain

su(Hw) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
C terminus
sufficient binding region
aa 363-941
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

(Kurshakova et al., 2007)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Kurshakova et al., 2007)
FBrf0201920-2.Y2H
Reference
Kurshakova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

E(y)2

bait

fused to LexA DNA-binding domain

su(Hw) 

Su(Hw)

prey

fused to GAL4 activation domain

su(Hw) 
su(Hw) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
su(Hw) 
Zinc fingers
sufficient binding region
aa 363-620aa 435-781
su(Hw) 
Zinc fingers
necessary binding region
aa 598-620aa 435-496
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Kurshakova et al., 2007)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kurshakova et al., 2007)
FBrf0201920-3.PD.MW
Reference
Kurshakova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

GST tag

e(y)2 

E(y)2

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein; prey produced as recombinant fusion protein.

(Kurshakova et al., 2007)
FBrf0201920-4.coIP.WB
Reference
Kurshakova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

E(y)2

bait
su(Hw) 

Su(Hw)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurshakova et al., 2007)
FBrf0201920-5.coIP.WB
Reference
Kurshakova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait
e(y)2 

E(y)2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurshakova et al., 2007)
FBrf0201920-6.coIP.WB
Reference
Kurshakova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

E(y)2

bait
su(Hw) 

Su(Hw)

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Kurshakova et al., 2007)
FBrf0201920-7.coIP.WB
Reference
Kurshakova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

FLAG tag

e(y)2 

E(y)2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kurshakova et al., 2007)
External Crossreferences and Linkouts ( 0 )
References (1)