Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction increases upon depletion of zuc but reduced again in zuc depleted cells that are also piwi depleted.
Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction decreases upon deletion of piwi in the depleted zuc background. Mutant armi experiment carried out in zuc and armi depleted background.
Source was cell extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Gasz construct lacks the C terminal domain, coordinates relative to Gasz-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Y551A; MID mutant lacking 5\' end piRNA binding; coordinates relative to piwi-PA
Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.
