FlyBase Gene Report: Dmel\armi

General Information

Symbol

Dmel\armi

Species

D. melanogaster

Name

armitage

Annotation Symbol

CG11513

Feature Type

protein_coding_gene

FlyBase ID

FBgn0041164

Gene Model Status

Current

Stock Availability

17 publicly available

Enzyme Name (EC)

RNA helicase (3.6.4.13)

RNA 5'-3' helicase (5.6.2.5)

Gene Summary

armitage (armi) encodes a highly conserved cytosolic 5'-3' RNA helicase that is involved in biogenesis of PIWI-interacting RNAs (piRNAs), which suppress transposable elements in the fly germline. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

63E1-63E1

Sequence location

3L:3,461,317..3,466,314 [+]

Recombination map

3-8

RefSeq locus

NT_037436 REGION:3461317..3466314

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (29 terms)

Molecular Function (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables 5'-3' RNA helicase activity

inferred from mutant phenotype

(Ge et al., 2019)

inferred from direct assay

(Pandey et al., 2017)

enables ATP hydrolysis activity

inferred from mutant phenotype

(Ge et al., 2019)

enables piRNA binding

inferred from mutant phenotype

(Yamashiro et al., 2020, Ge et al., 2019)

inferred from physical interaction with FLYBASE:lncRNA:flam; FB:FBgn0267704

(Munafò et al., 2019)

inferred from direct assay

(Yamashiro et al., 2020)

enables protein binding

inferred from physical interaction with FLYBASE:fs(1)Yb; FB:FBgn0000928

(Handler et al., 2011)

inferred from physical interaction with FLYBASE:piwi; FB:FBgn0004872

(Handler et al., 2011)

inferred from physical interaction with FLYBASE:vret; FB:FBgn0263143

(Handler et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables 5'-3' RNA helicase activity

inferred from sequence or structural similarity with UniProtKB:Q9HCE1

(Gene Ontology Curators, 2002-)

enables helicase activity

inferred from electronic annotation with InterPro:IPR041677

(InterPro Project Members, 2004-)

enables RNA binding

inferred from biological aspect of ancestor with PANTHER:PTN008507816

(GO Reference Genome Project, 2011-)

enables RNA helicase activity

non-traceable author statement

(Tomari et al., 2004)

Biological Process (13 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

involved_in dorsal appendage formation

inferred from mutant phenotype

(Klattenhoff et al., 2007)

involved_in embryonic axis specification

inferred from mutant phenotype

(Cook et al., 2004)

involved_in germarium-derived egg chamber formation

inferred from mutant phenotype

(Atikukke et al., 2014)

involved_in long-term memory

inferred from mutant phenotype

(Ashraf et al., 2006)

involved_in microtubule organizing center organization

inferred from mutant phenotype

(Klattenhoff et al., 2007)

involved_in negative regulation of DNA damage checkpoint

inferred from genetic interaction with FLYBASE:lok; FB:FBgn0019686

(Atikukke et al., 2014)

involved_in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay

inferred from mutant phenotype

(Cook et al., 2004)

involved_in oogenesis

inferred from mutant phenotype

(Hayashi et al., 2014)

involved_in pole plasm protein localization

inferred from mutant phenotype

(Klattenhoff et al., 2007)

involved_in primary piRNA processing

inferred from mutant phenotype

(Yamashiro et al., 2020, Ge et al., 2019, Sienski et al., 2015, Czech et al., 2013, Olivieri et al., 2012)

inferred from physical interaction with FLYBASE:fs(1)Yb; FB:FBgn0000928

(Hirakata et al., 2019)

involved_in regulatory ncRNA-mediated post-transcriptional gene silencing

inferred from mutant phenotype

(Tomari et al., 2004)

involved_in RISC complex assembly

inferred from mutant phenotype

(Tomari et al., 2004)

involved_in secondary piRNA processing

inferred from mutant phenotype

(Chang et al., 2019, Ge et al., 2019)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulatory ncRNA-mediated post-transcriptional gene silencing

inferred from biological aspect of ancestor with PANTHER:PTN000094957

(GO Reference Genome Project, 2011-)

Cellular Component (9 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Ashraf et al., 2006)

is_active_in cytoplasmic side of mitochondrial outer membrane

inferred from direct assay

(Munafò et al., 2019)

located_in cytoplasm

inferred from direct assay

(Saito et al., 2010)

inferred from direct assay

(Cook et al., 2004)

is_active_in cytosol

inferred from direct assay

(Huang et al., 2014)

located_in cytosol

inferred from direct assay

(Yamashiro et al., 2020)

located_in dendrite

inferred from direct assay

(Ashraf et al., 2006)

is_active_in extrinsic component of mitochondrial outer membrane

inferred from direct assay

(Ge et al., 2019, Huang et al., 2014)

located_in neuronal cell body

inferred from direct assay

(Ashraf et al., 2006)

is_active_in P granule

inferred from direct assay

(Munafò et al., 2019, Huang et al., 2014)

located_in P granule

inferred from direct assay

(Ge et al., 2019)

is_active_in Yb body

inferred from direct assay

(Hirakata et al., 2019, Munafò et al., 2019, Huang et al., 2014)

located_in Yb body

inferred from mutant phenotype

(Yamashiro et al., 2020)

inferred from direct assay

(Olivieri et al., 2012, Olivieri et al., 2010, Saito et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN000094957

(GO Reference Genome Project, 2011-)

is_active_in P granule

inferred from biological aspect of ancestor with PANTHER:PTN000094957

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UPF1-LIKE RNA HELICASES

Protein Family (UniProt)

Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily. (Q6J5K9)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

5'-3' RNA helicase activity

n ATP + n H2O + wound RNA = n ADP + n phosphate + unwound RNA (5.6.2.5)

RNA helicase activity

ATP + H2O = ADP + phosphate + H(+) (3.6.4.13)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Snapshot

Gene Group (FlyBase)

UPF1-LIKE RNA HELICASES -: RNA helicases of helicase superfamily SF1 are also referred to as UPF1-like helicases owing to their similarity with the prototype member, UPF1. SF1 family helicases are thought to mediate their effect by translocation on the nucleic acid, translocating in the 5'-3' direction. UPF1 is a central component of the nonsense mediated mRNA decay (NMD) pathway, which involves the step-wise assembly and disassembly of several protein factors to mediate target mRNA degradation. In the NMD pathway, UPF1 employs its catalytic activity to remodel mRNPs containing premature termination codons. (Adapted from PMID:29378013.)

Protein Function (UniProtKB)

Probable RNA helicase required for axial polarization of the oocyte during early and mid oogenesis (PubMed:15035984). Plays a central role in RNA interference (RNAi) process, a process that mediates mRNA destruction of translational repression (PubMed:15035984). Required for the assembly of the RISC complex, a complex required for target RNA destruction or repression (PubMed:15035985). May be required in the RISC assembly to unwind miRNAs, in the production of single-stranded miRNA from the double-stranded miRNA, a key step in RISC formation (PubMed:15035985). Required both for the translational control of oskar (osk) mRNA and cytoskeletal polarization in the oocyte (PubMed:15035984). Required for somatic primary piRNA biogenesis (PubMed:20966047). Involved in repression of long interspersed nuclear elements (LINEs) including HeT-A, I-element and TART LINEs (PubMed:17428915).

(UniProt, Q6J5K9)

Summary (Interactive Fly)

RNA helicase involved in posttranscriptional gene silencing - required for primary piRNA biogenesis - mutations disrupt mRNA translational silencing of oskar in the oocyte and silencing of Stellate in male germ cells

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\armi for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q6J5K9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.41

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Gene model reviewed during 6.32

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

armi-RB

FBtr0100641

NM_001014556

4145

1188

armi-RC

FBtr0309840

NM_001259674

3631

1154

armi-RD

FBtr0309841

NM_139559

4151

1188

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

armi-PB

FBpp0100102

135.7

1188

6.09

FBpp0301574

131.5

1154

6.25

FBpp0301575

135.7

1188

6.09

Q6J5K9

NP_647816

AAF47775

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1188 aa isoforms: armi-PB, armi-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Forms a complex with piwi and fs(1)Yb; this interaction is required for proper piRNA loading and nuclear localization of piwi. The interaction of piwi and fs(1)Yb is likely to occur via armi.

(UniProt, Q6J5K9)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\armi using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.11

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 13 -- 16

gonad

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

nurse cell of ovary

• cytoplasm

(Saito et al., 2010)

follicle cell of ovary

• cytoplasm & Yb body

(Saito et al., 2010)

somatic cell of ovariole of germarium

(Minakhina et al., 2014)

oogenesis & adult stage | female

female germline cell of germarium

(Minakhina et al., 2014)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

ovary

(Saito et al., 2010)

Additional Descriptive Data

Weak expression of armi is seen in the cytoplasm of germline nurse cells and their surrounding somatic follicle cells, which contain one or more strong foci of armi staining. Colocalization with fs(1)Yb suggests that the armi foci are Yb bodies. armi is only weakly associated with P granules.

(Saito et al., 2010)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Ashraf et al., 2006)

is_active_in cytoplasmic side of mitochondrial outer membrane

inferred from direct assay

(Munafò et al., 2019)

located_in cytoplasm

inferred from direct assay

(Saito et al., 2010)

inferred from direct assay

(Cook et al., 2004)

is_active_in cytosol

inferred from direct assay

(Huang et al., 2014)

located_in cytosol

inferred from direct assay

(Yamashiro et al., 2020)

located_in dendrite

inferred from direct assay

(Ashraf et al., 2006)

is_active_in extrinsic component of mitochondrial outer membrane

inferred from direct assay

(Ge et al., 2019, Huang et al., 2014)

located_in neuronal cell body

inferred from direct assay

(Ashraf et al., 2006)

is_active_in P granule

inferred from direct assay

(Munafò et al., 2019, Huang et al., 2014)

located_in P granule

inferred from direct assay

(Ge et al., 2019)

is_active_in Yb body

inferred from direct assay

(Hirakata et al., 2019, Munafò et al., 2019, Huang et al., 2014)

located_in Yb body

inferred from mutant phenotype

(Yamashiro et al., 2020)

inferred from direct assay

(Olivieri et al., 2012, Olivieri et al., 2010, Saito et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\armi in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\armi

View exonic expression by tissue for Dmel\armi

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0041164

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38427/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0041164

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0041164

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0041164

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11513

Flygut - An atlas of the Drosophila adult midgut

FBgn0041164

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\armi

Stages(s) 1-3
Stages(s) 4-6

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 16 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 25 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of armi

Transgenic constructs containing regulatory region of armi

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

Df(3L)AJ14

(Althoff et al., 2009)

Df(3L)armi^Δ1

(Olivieri et al., 2010)

Df(3L)armi-J

(Atikukke et al., 2014)

Df(3L)E1

(Cook et al., 2004)

Df(3L)Exel6095

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(3L)CRIMIC-CR70238

(Gene Disruption Project members, 2018-)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | maternal effect (with armi¹)

armi^72.1

lethal - all die during embryonic stage | maternal effect (with armi^72.1)

armi¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

armi^GD7319

viable, with Scer\GAL4^Mef2.PR

armi^GD7319

viable, with Scer\GAL4^pnr-MD237

armi^GD7319

viable, with Scer\GAL4^tin.CΔ4

armi^GD7319

Sterility

Allele

female semi-sterile, with Df(3L)Exel6095/CycJ^null

armi^+t6.3

female sterile

female sterile, with Scer\GAL4^nanos.PG

female sterile, with Scer\GAL4^{VP16.nanos.UTR}

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

armi^RNAi.UASp

fertile

armi^+t6

male semi-fertile, with Scer\GAL4^{VP16.nanos.UTR}

armi^GL00254

male semi-fertile (with armi¹)

armi^72.1

male semi-fertile (with armi^72.1)

armi¹

male semi-sterile

Other Phenotypes

Allele

abnormal memory (with Df(3L)E1)

armi^72.1

abnormal memory | recessive

armi^72.1

flightless, with Scer\GAL4^Mef2.PR

armi^GD7319

some die during embryonic stage | maternal effect (with armi¹)

armi^72.1

some die during embryonic stage | maternal effect (with armi^72.1)

armi¹

Phenotype manifest in

Allele

dorsal appendage, with CycJ^+t4

armi^null

dorsal appendage (with armi¹)

armi^72.1

dorsal appendage (with armi^72.1)

armi¹

dorsal appendage (with Df(3L)armi-J)

armi^72.1

dorsal appendage | maternal effect

dorsal appendage | maternal effect (with armi¹)

armi^72.1

dorsal appendage | maternal effect (with armi^72.1)

armi¹

egg, with CycJ^+t4

armi^null

egg | maternal effect

armi^72.1

egg | maternal effect (with Df(3L)E1)

egg chamber, with CycJ^+t4

armi^null

follicle cell (with Df(3L)E1)

armi^11R3

microtubule organizing center & oocyte (with armi¹)

armi^72.1

microtubule organizing center & oocyte (with armi^72.1)

armi¹

oocyte, with CycJ^+t4

armi^null

oocyte & microtubule

armi^72.1

oocyte & microtubule organizing center

armi^72.1

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (11)

Hsap\MOV10L1

10 of 14

Yes

Hsap\MOV10

9 of 14

Yes

Hsap\SETX

4 of 14

Hsap\CT55

3 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (10)

Rnor\Mov10l1

9 of 14

Yes

Rnor\Mov10

9 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

Yes

Rnor\LOC100360296

1 of 14

Yes

Rnor\Zgrf1

1 of 14

Mus musculus (laboratory mouse) (12)

Mmus\Mov10l1

9 of 14

Yes

Mmus\Mov10

9 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

Yes

Mmus\Ct55

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (10)

Xtro\mov10l1

8 of 13

Yes

Xtro\mov10

5 of 13

Yes

Xtro\XB5951253

5 of 13

Yes

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (12)

Drer\mov10a

10 of 14

Yes

Drer\mov10b.1

10 of 14

Yes

Drer\mov10b.2

10 of 14

Yes

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Drer\helz2c

1 of 14

Drer\zgc:154086

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\eri-7

6 of 14

Yes

Cele\Y106G6D.5

4 of 14

Yes

Cele\C41D11.6

3 of 14

Yes

Cele\C44H9.4

3 of 14

Yes

Cele\smg-2

3 of 14

Cele\C05C10.2

1 of 14

Yes

Cele\dna-2

1 of 14

Cele\emb-4

1 of 14

Cele\F53E10.5

1 of 14

Yes

Cele\K08D10.5

1 of 14

Yes

Cele\R03D7.2

1 of 14

Cele\Y53F4B.10

1 of 14

Yes

Cele\znfx-1

1 of 14

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP006939

12 of 12

Yes

4 of 12

4 of 12

3 of 12

3 of 12

2 of 12

1 of 12

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (18)

Atha\SDE3

11 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\SEN1

4 of 13

Yes

3 of 13

3 of 13

2 of 13

2 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\upf1

4 of 12

Yes

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:armi. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

7 of 13

7 of 13

6 of 13

6 of 13

5 of 13

4 of 13

4 of 13

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

primary ovarian insufficiency (postulated), MOV10-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

armi¹

model of primary ovarian insufficiency

CEA

(Bestetti et al., 2021)

CEA with armi^KG04664

(Bestetti et al., 2021)

armi^KG04664

model of primary ovarian insufficiency

CEA

(Bestetti et al., 2021)

CEA with armi¹

(Bestetti et al., 2021)

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

MOV10L1; Mov10 like RNA helicase 1

model of spermatogenic failure 73

IEA

(FlyBase, 2019-)

CT55; cancer/testis antigen 55

model of X-linked spermatogenic failure 7

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MOV10L1; Mov10 like RNA helicase 1

10 of 14

605794

SPERMATOGENIC FAILURE 73; SPGF73

spermatogenic failure 73

MOV10; Mov10 RNA helicase

9 of 14

MOV10 RISC COMPLEX RNA HELICASE ; MOV10

SETX; senataxin

4 of 14

SENATAXIN; SETX

CT55; cancer/testis antigen 55

3 of 14

301105

SPERMATOGENIC FAILURE, X-LINKED, 7; SPGFX7

X-linked spermatogenic failure 7

HELZ; helicase with zinc finger

3 of 14

606699

UPF1; UPF1 RNA helicase and ATPase

3 of 14

UPF1 RNA HELICASE AND ATPase; UPF1

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

armi

anti tag coimmunoprecipitation, western blot

(Olivieri et al., 2010)

armi - AGO3

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Huang et al., 2014)

armi - Gasz

anti bait coimmunoprecipitation, western blot, pull down, anti tag coimmunoprecipitation, anti tag western blot

(Yamashiro et al., 2020, Munafò et al., 2019)

armi - SoYb

anti bait coimmunoprecipitation, western blot

(Hirakata et al., 2019)

armi - Tdrd3

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry

(Lee et al., 2025)

armi - daed

anti tag coimmunoprecipitation, anti tag western blot

(Munafò et al., 2019)

armi - fs(1)Yb

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, proximity-dependent biotin identification, Identification by mass spectrometry, anti tag western blot

(Munafò et al., 2021, Hirakata et al., 2019, Ishizu et al., 2019, Handler et al., 2011, Qi et al., 2011, Olivieri et al., 2010, Saito et al., 2010)

armi - piwi

anti bait coimmunoprecipitation, western blot, affinity chromatography technology, Identification by mass spectrometry, anti tag western blot, anti tag coimmunoprecipitation

(Lee et al., 2025, Yamashiro et al., 2020, Munafò et al., 2019, Ilyin et al., 2017, Pandey et al., 2017, Handler et al., 2011, Olivieri et al., 2010, Saito et al., 2010)

armi - shu

anti bait coimmunoprecipitation, western blot

(Olivieri et al., 2012)

armi - vas

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry

(Durdevic and Ephrussi, 2019)

armi - vret

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Hirakata et al., 2019, Handler et al., 2011, Zamparini et al., 2011)

armi - zuc

anti tag coimmunoprecipitation, protein cross-linking with a bifunctional reagent, western blot, anti bait coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Ge et al., 2019, Ishizu et al., 2019)

RNA-protein

Physical Interaction

Assay

References

armi - lncRNA:flam

clip-seq, clip

(Ishizu et al., 2019, Munafò et al., 2019)

armi - tj

clip-seq, clip

(Ishizu et al., 2019, Munafò et al., 2019)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

armi

suppressible

lok

(Atikukke et al., 2014, Orsi et al., 2010, Klattenhoff et al., 2007)

CycJ|armi

suppressible

lok

(Atikukke et al., 2014)

armi

suppressible

mei-41

(Klattenhoff et al., 2009, Klattenhoff et al., 2007)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Gas41

enhanceable

armi

(Gandhi et al., 2015)

piwi

enhanceable

CycJ|armi

(Atikukke et al., 2014)

External Data

Subunit Structure (UniProtKB)

Forms a complex with piwi and fs(1)Yb; this interaction is required for proper piRNA loading and nuclear localization of piwi. The interaction of piwi and fs(1)Yb is likely to occur via armi.

(UniProt, Q6J5K9 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0041164

MIST (genetic) - An integrated Molecular Interaction Database

38427

MIST (protein-protein) - An integrated Molecular Interaction Database

38427

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_dicistronic_mRNA

(FlyBase Genome Annotators, 2020)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-8

Cytogenetic map

63E1-63E1

Sequence location

3L:3,461,317..3,466,314 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63E1-63E1

Limits computationally determined from genome sequence between P{PZ}kst⁰¹³¹⁸ and P{PZ}Sc2⁰⁵⁶³⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-8

(FlyBase, 2016)

3-6.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (17)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

8514

y¹ w^*; P{UASp-armi-EGFP}3

31604

y¹ v¹; P{TRiP.JF01388}attP2

98880

y¹ w¹; P{TKO-UAS.GS06023}attP40/CyO

8544

w^*; armi^72.1/TM6C, Sb¹ Tb¹

8513

y¹ w^*; P{lacW}armi¹/TM3, Sb¹

8515

w^*; P{UASp-armi-EGFP}2/CyO; Ki¹/TM3, Ser¹

35343

y¹ sc^* v¹ sev²¹; P{TRiP.GL00254}attP2

Kyoto

105322

y^* w^*; P{GawB}armi^NP6679

NIG-Fly

11513R-2

P{NIG.11513R}2

HMS00098

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00098}attP2

VDRC

v103589

P{KK101517}VIE-260B

v16206

w¹¹¹⁸; P{GD7319}v16206

More stocks available...

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (27)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

IP15135

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0041164

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0041164

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Chary and Hayashi, 2023, Saito et al., 2010)

polyclonal

(Huang et al., 2014)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

armi is required for the accumulation of repeat-associated small interfering RNAs (rasiRNAs).

(Vagin et al., 2006)

armi is required for RNA interference, and the data suggests that armi is required for the assembly of siRNA into functional RNA-induced silencing complex (RISC).

(Tomari et al., 2004)

armi mutants are impaired in a step in RNA-induced silencing complex (RISC) assembly after binding of the siRNA to the Dcr-2/r2d2 protein heterodimer.

(Tomari et al., 2004)

armi is required for posterior and dorsoventral patterning during oogenesis.

(Cook and Theurkauf, 2000)

Mutations in armi block posterior migration of the microtubule organising centre and cause defects in RNA localisation in the oocyte.

(Cook and Theurkauf, 2000)

Relationship to Other Genes

Source for database merge of

Source for merge of: armi CG11513

(Cook et al., 2004)

Additional comments

One or more of the processed transcripts for this gene contain(s) two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG46463 and CG11513.

(FlyBase Genome Annotators, 2020)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

The gene is named "armitage" after the navigator on Robert Falcon Scott's failed Discovery expedition to the South Pole.

(Cook et al., 2004)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

APC16

(Hutchins et al., 2010)

ARMI

(Gamez et al., 2020, Kolaczkowski et al., 2011)

CG11513

(Na et al., 2016, Schertel et al., 2013, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Ni et al., 2008, Kai et al., 2005, Anantharaman et al., 2002)

armi

(Cacchione et al., 2025, Fitz-James et al., 2025, Nieken et al., 2023, Venkei et al., 2023, Ding et al., 2022, Kanca et al., 2022, Bestetti et al., 2021, Ellison et al., 2020, FlyBase Genome Annotators, 2020, Saint-Leandre et al., 2020, Takahashi et al., 2020, Wang et al., 2020, Chang et al., 2019, Ge et al., 2019, Kolliopoulou et al., 2019, Story et al., 2019, Whittle and Extavour, 2019, Gene Disruption Project members, 2018-, Dufourt et al., 2017, Rojas-Ríos et al., 2017, Transgenic RNAi Project members, 2017-, Vrettos et al., 2017, Blumenstiel et al., 2016, Brewer-Jensen et al., 2016, Carbone et al., 2016, Catrina et al., 2016, Fagegaltier et al., 2016, Ge et al., 2016, Hur et al., 2016, Lee et al., 2016, Na et al., 2016, Vilmos et al., 2016, Gandhi et al., 2015, Han et al., 2015, Onorati et al., 2015, Yu et al., 2015, Atikukke et al., 2014, Hayashi et al., 2014, Hayashi et al., 2014, Luck et al., 2014, Ma et al., 2014, Minakhina et al., 2014, Zhang et al., 2014, Czech et al., 2013, Dennis et al., 2013, Dönertas et al., 2013, Handler et al., 2013, Kwon et al., 2013, Muerdter et al., 2013, Ohtani et al., 2013, Schertel et al., 2013, Terriente-Felix et al., 2013, Vagin et al., 2013, Gistelinck et al., 2012, Hodgetts et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Kelleher et al., 2012, Olivieri et al., 2012, Sienski et al., 2012, Soshnev et al., 2012, Vazquez-Pianzola and Suter, 2012, Handler et al., 2011, Klenov et al., 2011, Ni et al., 2011, Haase et al., 2010, Khurana et al., 2010, Nagao et al., 2010, Olivieri et al., 2010, Orsi et al., 2010, Rouget et al., 2010, Yassin et al., 2010, Althoff et al., 2009, Klattenhoff et al., 2009, Malone et al., 2009, Navarro et al., 2009, Robine et al., 2009, Vilmos et al., 2009, Chambeyron et al., 2008, Atikukke and Finley, 2007, Josse et al., 2007, Klenov et al., 2007, Lim and Kai, 2007, Tadros et al., 2007, Ashraf et al., 2006, Liebl et al., 2006, Shigenobu et al., 2006, Vagin et al., 2006, Vagin et al., 2006, Liebl and Featherstone, 2005)

Name Synonyms

Secondary FlyBase IDs

FBgn0035448

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 46 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38427

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q6J5K9

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q6J5K9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9NDG7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q6J5K9

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0041164

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38427/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0041164

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0041164

FlyMine - An integrated database for Drosophila genomics

FBgn0041164

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11513

MARRVEL_MODEL - MARRVEL (model organism gene)

38427

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0041164

DroID - A comprehensive database of gene and protein interactions.

FBgn0041164

DRSC - Results frm RNAi screens

FBgn0041164

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0041164

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0041164

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11513

Flygut - An atlas of the Drosophila adult midgut

FBgn0041164

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC010546

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

38427

MIST (protein-protein) - An integrated Molecular Interaction Database

38427

References (223)

The following external sites may use different assemblies or annotations than FlyBase.

0.11

Report Sections