FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\CycJ+t4
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General Information
Symbol
Dmel\CycJ+t4
Species
D. melanogaster
Name
FlyBase ID
FBal0299629
Feature type
allele
Associated gene
Dmel\CycJ
Associated Insertion(s)
Carried in Construct
P{CycJ+t4}
Key Links
Genomic Maps

Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Atikukke et al., 2014)
Progenitor genotype
Carried in construct
P{CycJ+t4}
Cytology
Description

A genomic fragment containing a 4kb region corresponding to CG14971 from 1.1kb upstream of the ATG to 1.9kb downstream of the stop codon, including the last exon of armi and 400bp downstream of the CG14971 stop codon (amplified from the BAC clone DS01105).

(Atikukke et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
CycJ
Encoded product / tool
CycJ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
rescue_region
3L:3,465,385..3,469,292 [+]
Linked to:
BglII-BglII restriction fragment
Comment:

3.9 kb BglII fragment contains CycJ sequences from 1.1kb upstream of the ATG to 1.9kb downstream of the stop codon.

(Atikukke et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      CycJ+t4 is a suppressor | partially of female sterile phenotype of Df(3L)Exel6095/CycJnull, armi+t6.3, aubHN2/aubQC42

      (Atikukke et al., 2014)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      CycJ+t4, arminull has dorsal appendage phenotype, suppressible by lokp6

      (Atikukke et al., 2014)
      NOT Enhancer of
      Statement
      Reference

      CycJ+t4, armi+t6.3, Df(3L)Exel6095, CycJnull is a non-enhancer of egg chamber phenotype of Df(2L)BSC145/piwi06843

      (Atikukke et al., 2014)
      Suppressor of
      Statement
      Reference

      CycJ+t4 is a suppressor of egg chamber phenotype of Df(3L)Exel6095/CycJnull, armi+t6.3, aubHN2/aubQC42

      (Atikukke et al., 2014)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of CG14971t4 does not further enhance the rescue of the Df(3L)armi-J mutant oogenesis defects by CycJ+t4.

      Co-expression of CycJ+t4 further enhances the rescue of either Df(3L)armi-J or Df(3L)armi-J/Df(3L)Exel6095 mutant oogenesis defects by armi+t6.3. The hatching rate is improved from 30% to 73-76%.

      A CycJnull/Df(3L)Exel6095 mutant background (expressing armi+t6.3 to restore armi[+] function) enhances the oogenesis defects seen in piwi06843/Df(2L)BSC145 mutant females. The frequency of compound egg chambers (multiple cysts in one follicular epithelium) is comparable to that seen in Df(3L)armi-J. Addition of CycJ+t4 decreases the frequency of compound egg chambers to the levels seen in the piwi mutant alone.

      CycJnull/Df(3L)Exel6095 (expressing armi+t6.3 to restore armi[+] function) enhances the fertility defects seen in aubHN2/aubQC42 mutant females. The resulting flies lay no eggs and oocyte development is arrested, with egg chambers rarely advancing beyond stage 7-8. Compound egg chambers (multiple cysts in a single follicular epithelium) are seen, and these often include a disorganised layer of follicle cells almost separating two fully developed cysts. This phenotype can be fully suppressed by expression of CycJ+t4.

      (Atikukke et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments

      Expression of CycJ+t4 significantly rescues the oogenesis defects seen in homozygous Df(3L)armi-J females. Females are capable of producing fully developed stage 14 egg chambers and of laying eggs, albeit at a dramatically reduced rate compared to heterozygotes. There is also a dramatic decrease in the frequency of compound egg chambers (multiple cysts in one follicular epithelium) when CycJ+t4 is expressed in Df(3L)armi-J/Df(3L)Exel6095.

      (Atikukke et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      CycJ+t4
      Name Synonyms
      Secondary FlyBase IDs
        References (1)