FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ALiX, tum
FlyBase ID
FBig0000120160
Interaction Network
Interactions Browser links
ALiX network
tum network
Reported Interactions
FBrf0243783-2.coIP.WB
Reference
Lie-Jensen et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
ALiX 

ALIX

bait

FLAG tag

ALiX 
tum 

Tum

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ALiX 
V domain
sufficient binding region
aa 362-708
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lie-Jensen et al., 2019)
FBrf0243783-4.PD.A
Reference
Lie-Jensen et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ALiX 

ALIX

bait

GST tag

tum 

Tum

prey

Myc tag, [35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction is weak.

(Lie-Jensen et al., 2019)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Lie-Jensen et al., 2019)
FBrf0243783-8.coIP.MS
Reference
Lie-Jensen et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
ALiX 

ALIX

bait

GFP tag

ALiX 
tum 

Tum

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ALiX 
V domain
sufficient binding region
aa 362-708
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lie-Jensen et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)