Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).
Q26A,F27A,I29A, coordinates relative to smt3-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.
Q26A,F27A,I29A, coordinates relative to smt3-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
V465A, L467A; coordinates relative to Su(var)2-10-PJ
L319A, V321A, L323A; coordinates relative to Su(var)2-10-PJ
I398A, L400A; coordinates relative to Su(var)2-10-PJ
Mutation of Su(var)2-10 hydrophobic amino acids in the described domains, disrupts or decreases interaction with Sumo. Addition of piwi Mid domain decreases interaction.
Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.
I398A, L400A; coordinates relative to Su(var)2-10-PJ
Positive control.
Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.
