FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sbr, nxf2
FlyBase ID
FBig0000120269
Interaction Network
Interactions Browser links
sbr network
nxf2 network
Reported Interactions
FBrf0244040-21.coIP.WB
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

bait

GFP tag

nxf2 
sbr 

dNxf1

prey

Halo tag

sbr 
sbr 
sbr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sbr 
NTF2 domain
necessary binding region
aa 366-545

coordinates relative to sbr-PA

nxf2 
NTF2 domain
sufficient binding region
aa 555-841

coordinates relative to nxf2-PA

sbr 
NTF2 domain
sufficient binding region
aa 359-612

coordinates relative to sbr-PA

sbr 
UBA domain
sufficient binding region
aa 601-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhao et al., 2019)
FBrf0244040-22.coIP.WB
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

bait

GFP tag

nxf2 
sbr 

dNxf1

prey

Halo tag

sbr 
sbr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
NTF2 domain
sufficient binding region
aa 555-841

coordinates relative to nxf2-PA

sbr 
NTF2 domain
sufficient binding region
aa 359-612

coordinates relative to sbr-PA

sbr 
UBA domain
sufficient binding region
aa 601-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhao et al., 2019)
FBrf0244040-23.coIP.WB
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
sbr 

dNxf1

bait

GFP tag

sbr 
sbr 
nxf2 

dNxf2

prey

Halo tag

nxf2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
NTF2 domain
sufficient binding region
aa 555-841

coordinates relative to nxf2-PA

sbr 
NTF2 domain
sufficient binding region
aa 359-612

coordinates relative to sbr-PA

sbr 
UBA domain
sufficient binding region
aa 601-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhao et al., 2019)
FBrf0244040-24.SLC
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
sbr 

dNxf1

bait

Gluc1 tag

sbr 
nxf2 

dNxf2

prey

Gluc2 tag

nxf2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
NTF2 domain
sufficient binding region
aa 555-841

coordinates relative to nxf2-PA

sbr 
C-terminal domain
sufficient binding region
aa 346-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Zhao et al., 2019)
FBrf0244040-25.SLC
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
nxf2 

dNxf2

bait

Gluc1 tag

nxf2 
sbr 

dNxf1

prey

Gluc2 tag

sbr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nxf2 
NTF2 domain
sufficient binding region
aa 555-841

coordinates relative to nxf2-PA

sbr 
C-terminal domain
sufficient binding region
aa 346-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Zhao et al., 2019)
FBrf0244040-27.PD.MW
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sbr 

dNxf1

bait

GST tag

sbr 
sbr 
nxf2 

dNxf2

prey
nxf2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sbr 
NTF2 domain
sufficient binding region
aa 359-544

coordinates relative to sbr-PA

sbr 
UBA domain
sufficient binding region
aa 601-672

coordinates relative to sbr-PA

nxf2 
NTF2 domain
sufficient binding region
aa 555-772

coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The sbr-nxf2 interaction may not be direct as Nxt1 was also added to the pull-down assay.

(Zhao et al., 2019)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhao et al., 2019)
FBrf0244040-30.CL.MS
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sbr 

dNxf1

neutral component
sbr 

dNxf1

neutral component
sbr 
sbr 

dNxf1

neutral component
sbr 
nxf2 

dNxf2

neutral component
nxf2 
nxf2 

dNxf2

neutral component
nxf2 

dNxf2

neutral component
nxf2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sbr 
NTF2 and UBA domains
sufficient binding region
aa 359-672

coordinates relative to sbr-PA

nxf2 
NTF2 domain
sufficient binding region
aa 555-772

coordinates relative to nxf2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Zhao et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)