FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eIF4G1, Mkrn1
FlyBase ID
FBig0000120318
Interaction Network
Interactions Browser links
eIF4G1 network
Mkrn1 network
Reported Interactions
FBrf0244799-3.coIP.WB
Reference
Dold et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
eIF4G1 

eIF4G

bait

Myc tag

Mkrn1 

Mkrn1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dold et al., 2020)

Mkrn1 with a RING domain mutation to increase protein stability was used in interaction assays.

(Dold et al., 2020)
FBrf0244799-6.coIP.WB
Reference
Dold et al., 2020
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4G1 

eIF4G

bait
Mkrn1 

Mkrn1

prey

Venus tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Dold et al., 2020)
FBrf0244799-9.coIP.MS
Reference
Dold et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Mkrn1 

Mkrn1

bait

Myc tag

eIF4G1 

eIF4G

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Both wildtype Mkrn1 and Mkrn1 with a RING domain mutation to increase protein stability were used in interaction assays.

(Dold et al., 2020)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Dold et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)