FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Rab11, spn-F
FlyBase ID
FBig0000120416
Interaction Network
Interactions Browser links
Rab11 network
spn-F network
Reported Interactions
FBrf0245011-1.coIP.WB
Reference
Lin et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rab11 

Rab11

bait
spn-F 

SpnF

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Lin et al., 2020)
FBrf0245011-2.coIP.WB
Reference
Lin et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
spn-F 

SpnF

bait

FLAG tag

Rab11 

Rab11

prey

Myc tag

Rab11 
Rab11 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab11 
interaction domain
mutation decreasing interaction
aa 70

constitutively active (CA) Q70L, coordinates relative to Rab11-PA

Rab11 
interaction domain
mutation increasing interaction
aa 25

dominant negative (DN) S25N, coordinates relative to Rab11-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lin et al., 2020)
FBrf0245011-3.coIP.WB
Reference
Lin et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rab11 

Rab11

bait

FLAG tag

spn-F 

SpnF

prey

Myc tag

spn-F 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spn-F 
SCD domain
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lin et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)