Miga, Vap33 Physical Interaction report

Identification by mass spectrometry

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Miga

bait

FLAG, HA tag

Vap33

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Xu et al., 2020)

FBrf0246278-2.coIP.WB

Reference

Xu et al., 2020

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Miga

bait

V5 tag

Miga

Vap33

prey

FLAG tag

Vap33

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Vap33

interaction domain

mutation decreasing interaction

aa 58

P58S, coordinates relative to Vap33-PA

Miga

FFAT-like domain

mutation disrupting interaction

aa 247,249aa 246,249

F247A,S249A, coordinates relative to Miga-PA

S246A,S249A, coordinates relative to Miga-PA

Miga

FFAT-like domain

sufficient binding region

aa 229-267

coordinates relative to Miga-PA

Miga

phosphorylation cluster V

mutation decreasing interaction

aa 225,228,230,233,237,243

S225A,S228A,S230A,S233A,S237A,S243A, coordinates relative to Miga-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 2020)

FBrf0246278-3.coIP.WB

Reference

Xu et al., 2020

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Vap33

bait

FLAG tag

Vap33

Miga

prey

V5 tag

Miga

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Vap33

interaction domain

mutation decreasing interaction

aa 58

P58S, coordinates relative to Vap33-PA

Miga

FFAT-like domain

mutation disrupting interaction

aa 247,249aa 246,249

F247A,S249A, coordinates relative to Miga-PA

S246A,S249A, coordinates relative to Miga-PA

Miga

FFAT-like domain

sufficient binding region

aa 229-267

coordinates relative to Miga-PA

Miga

phosphorylation cluster V

mutation decreasing interaction

aa 225,228,230,233,237,243

S225A,S228A,S230A,S233A,S237A,S243A, coordinates relative to Miga-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xu et al., 2020)

FBrf0246278-4.coIP.WB

Reference

Xu et al., 2020

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Miga

bait

HA tag

Vap33

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected genomic rescue fragment; prey produced from transfected construct.

(Xu et al., 2020)

FBrf0246278-5.coIP.WB

Reference

Xu et al., 2020

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Vap33

bait

FLAG tag

Miga

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected genomic rescue fragment.

(Xu et al., 2020)

FBrf0254939-15.coIP.WB

Reference

Xu et al., 2022

Description

physical association

Assay