Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was live S2R+ cells; bait and prey produced from endogenous genes.
bru1 was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.
