mod(mdg4), uno Physical Interaction report

Identification by mass spectrometry

Collection

Source/Stage

testis / wandering third instar larval stage pupal stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mod(mdg4)

mnm

bait

GFP tag

uno

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was testes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Weber et al., 2020)

FBrf0254845-4.coIP.WB

Reference

Kabakci et al., 2022

Description

physical association

Assay

western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

uno

bait

EGFP tag

mod(mdg4)

MNM

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kabakci et al., 2022)

MNM isoform of mod(mdg4).

(Kabakci et al., 2022)

FBrf0255412-13.coIP.WB

Reference

Kabakci et al., 2022

Description

physical association

Assay

western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

uno

UNO

bait

EGFP tag

mod(mdg4)

MNM

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Experiment carried out in cells also co-expressing SA2, mod(mdg4), and tef.

(Kabakci et al., 2022)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kabakci et al., 2022)

FBrf0255412-15.coIP.WB

Reference

Kabakci et al., 2022

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

uno

UNO

bait

EGFP tag

uno

mod(mdg4)

MNM

prey

mCherry tag

mod(mdg4)

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

uno

C terminal domain

sufficient binding region

aa 241-417

coordinates relative to uno-PA

mod(mdg4)

C terminal domain

sufficient binding region

aa 403-514

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Experiment carried out in cells also co-expressing tef.

(Kabakci et al., 2022)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kabakci et al., 2022)

FBrf0255412-3.coIP.WB

Reference

Kabakci et al., 2022

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

mod(mdg4)

MNM

bait

mCherry tag

uno

UNO

prey

EGFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kabakci et al., 2022)

FBrf0255412-7.coIP.WB

Reference

Kabakci et al., 2022

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

uno

UNO

bait

EGFP tag

uno

mod(mdg4)

MNM

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

uno

C terminal domain

sufficient binding region

aa 241-417

coordinates relative to uno-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kabakci et al., 2022)

FBrf0255412-8.coIP.WB

Reference

Kabakci et al., 2022

Description

physical association

Assay

Collection

Source/Stage

Cell line used