ctp, Panx Physical Interaction report

anti tag western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

ctp

Ctp

bait

FLAG tag

Panx

prey

HA tag

Panx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Panx

C-terminal domain

sufficient binding region

aa 261-541

coordinates relative to Panx-PA

Panx

C-terminal domain

necessary binding region

aa 261-541

coordinates relative to Panx-PA

Panx

TQT motif 1

mutation decreasing interaction

aa 462-465

TQTE to GGGK, coordinates relative to Panx-PA

Panx

TQT motif 2

mutation decreasing interaction

aa 475-478

TQVR to AGGG, coordinates relative to Panx-PA

Panx

TQT motifs 1 and 2

mutation disrupting interaction

aa 462-465,475-478

TQTE to GGGK,TQVR to AGGG, coordinates relative to Panx-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from transfected construct.

(Eastwood et al., 2021)

FBrf0248008-5.coIP.MS

Reference

Eastwood et al., 2021

Description

physical association

Assay

Collection

Source/Stage

Cell line used

isothermal titration calorimetry

Participants

Corresponds to

Reported as

Role

Note

ctp

Ctp

bait

FLAG tag

Panx

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eastwood et al., 2021)

FBrf0248008-6.ITC

Reference

Eastwood et al., 2021

Description

physical association

Assay

predetermined participant

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Panx

bait

Panx

ctp

Ctp

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Panx

C-terminal domain

sufficient binding region

unspecified

Panx

TQT motif 1

mutation decreasing interaction

aa 462-465

TQTE to GGGK, coordinates relative to Panx-PA

Panx

TQT motif 2

mutation decreasing interaction

aa 475-478

TQVR to AGGG, coordinates relative to Panx-PA

Panx

TQT motifs 1 and 2

mutation disrupting interaction

aa 462-465,475-478

TQTE to GGGK,TQVR to AGGG, coordinates relative to Panx-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Eastwood et al., 2021)

Kd = 0.01uM, Panx :ctp stoichiometry = 1:2

(Eastwood et al., 2021)

FBrf0248277-1.coIP.WB

Reference

Schnabl et al., 2021

Description

physical association

Assay

western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

ctp

Ctp

bait

GFP tag

Panx

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was extract of OSC cell line; bait produced from gene tagged at endogenous locus; prey produced from endogenous gene.

(Schnabl et al., 2021)

FBrf0248277-14.coIP.MS

Reference

Schnabl et al., 2021

Description

physical association

Assay

molecular weight estimation by staining

Collection

Source/Stage

ovary / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

ctp

Ctp

bait

GFP tag

Panx

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Schnabl et al., 2021)

FBrf0248277-21.CH.MW

Reference

Schnabl et al., 2021

Description

physical association

Assay

molecular sieving

light scattering

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Panx

neutral component

Panx

neutral component

Panx

neutral component

Panx

ctp

Ctp

neutral component

ctp

Ctp

neutral component

ctp

Ctp

neutral component

ctp

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Panx

ctp interaction site 1

sufficient binding region

aa 455-466

coordinates relative to Panx-PA

Panx

ctp interaction site 2

sufficient binding region

aa 467-480

coordinates relative to Panx-PA

ctp

interaction domain

sufficient binding region

aa 1-89

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

The molecular weights of the observed complexes correspond to a single ctp homodimer with two Panx peptides containing single ctp binding sites or two ctp homodimers with two Panx peptides containing both ctp binding sites.

(Schnabl et al., 2021)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Schnabl et al., 2021)

FBrf0248277-22.ITC

Reference

Schnabl et al., 2021

Description

physical association

Assay

isothermal titration calorimetry

predetermined participant

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

ctp

Ctp

bait

ctp

Panx

prey

Panx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Panx

ctp interaction site 1

sufficient binding region

aa 455-466

coordinates relative to Panx-PA, Kd = 75.8nM

Panx

ctp interaction site 2

sufficient binding region

aa 467-480

coordinates relative to Panx-PA, Kd = 138nM

Panx

C-terminal domain residues 462-464,475-476

mutation disrupting interaction

aa 462-464,475-476

TQT,TQ to A, coordinates relative to Panx-PA

ctp

interaction domain

sufficient binding region

aa 1-89

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Schnabl et al., 2021)

FBrf0248277-23.XC

Reference

Schnabl et al., 2021

Description

physical association

Assay

x-ray crystallography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Panx

neutral component

Panx

neutral component

Panx

neutral component

Panx

ctp

Ctp

neutral component

ctp

Ctp

neutral component

ctp

Ctp

neutral component

ctp

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Panx

ctp interaction site 1

sufficient binding region

aa 455-466

coordinates relative to Panx-PA

Panx

ctp interaction site 2

sufficient binding region

aa 467-480

coordinates relative to Panx-PA

ctp

interaction domain

sufficient binding region

aa 1-89

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Schnabl et al., 2021)

FBrf0248277-25.coIP.MS

Reference

Schnabl et al., 2021

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Panx

bait

GFP tag

ctp

Ctp

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was extract of OSC cell line; bait produced from gene tagged at endogenous locus; prey produced from endogenous gene.

(Schnabl et al., 2021)

FBrf0248277-3.coIP.MW

Reference

Schnabl et al., 2021

Description

physical association

Assay

molecular weight estimation by staining

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Panx

bait

GFP tag

ctp

Ctp

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from gene tagged at endogenous locus; prey produced from endogenous gene.

(Schnabl et al., 2021)

FBrf0248277-6.coIP.WB

Reference

Schnabl et al., 2021

Description

physical association

Assay

anti tag western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

Panx

bait

FLAG tag

Panx

ctp

Ctp

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Panx

C-terminal domain

sufficient binding region

aa 447-541

coordinates relative to Panx-PA

Panx

C-terminal domain residues 462-464,475-476

mutation disrupting interaction

aa 462-464,475-476

TQT,TQ to A, coordinates relative to Panx-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Schnabl et al., 2021)

FBrf0248277-9.coIP.MS

Reference

Schnabl et al., 2021

Description

physical association

Assay

Collection

Source/Stage

Cell line used