Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Step one in a two step sequential IP from S2 cells expressing aub, krimp and AGO3. The anti-FLAG(aub) immunoprecipitate was then subsequently immunoprecipitated with anti-GFP(krimp). The presence of AGO3 in the final precipitate suggests that one molecule of krimp binds aub and AGO3.

Kd = 8.26uM with R15me2 modified aub.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide source unspecified.

The aub peptide was R15me2 modified.

Interaction in vitro; krimp produced as a recombinant fusion protein in bacterial system; aub peptide source unspecified.