FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AMPKα, Parp1
FlyBase ID
FBig0000122365
Interaction Network
Interactions Browser links
AMPKα network
Parp1 network
Reported Interactions
FBrf0256083-1.coIP.MS
Reference
Guo et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AMPKα 

AMPKα

bait

GFP tag

Parp1 

Parp1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Guo et al., 2023)
FBrf0256083-2.coIP.WB
Reference
Guo et al., 2023
Description
physical association
Assay
Collection
Source/Stage
indirect flight muscle / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AMPKα 

AMPKα

bait
Parp1 

Parp1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was indirect flight muscles of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Guo et al., 2023)
FBrf0256083-3.coIP.WB
Reference
Guo et al., 2023
Description
physical association
Assay
Collection
Source/Stage
indirect flight muscle / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Parp1 

Parp1

bait
AMPKα 

AMPKα

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was indirect flight muscles of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Guo et al., 2023)
FBrf0256083-4.coIP.WB
Reference
Guo et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Parp1 

Parp1

bait

FLAG tag

AMPKα 

AMPKα

prey

GFP tag

AMPKα 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AMPKα 
interaction domain 3 residues 360-580
sufficient binding region
aa 360-580

coordinates relative to AMPKα-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Guo et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)