FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Arf1, Patronin
FlyBase ID
FBig0000122740
Interaction Network
Interactions Browser links
Arf1 network
Patronin network
Reported Interactions
FBrf0257549-1.BiFC.FM
Reference
Gujar et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Arf1 

Arf1

bait

Myc-YFP tag, N-terminal fragment

Arf1 
Patronin 

Patronin

prey

HA-YFP tag, C-terminal fragment

Patronin 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arf1 
interaction domain residue 71
necessary binding region
aa 71

Q71L; coordinates relative to Arf1-PA

Patronin 
CCK domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Gujar et al., 2023)

Patronin CCK domain interacts preferentially with the GDP-bound form of Arf1.

(Gujar et al., 2023)
FBrf0257549-2.BiFC.FM
Reference
Gujar et al., 2023
Description
physical association
Assay
Collection
Source/Stage
neuroblast / first instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arf1 

Arf1

bait

Myc-YFP tag, N-terminal fragment

Arf1 
Patronin 

Patronin

prey

HA-YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arf1 
interaction domain residue 71
mutation decreasing interaction
aa 71

Q71L; coordinates relative to Arf1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was neuroblasts from larva at 16hr ALH; proteins produced from transgenic constructs.

(Gujar et al., 2023)

Patronin interacts preferentially with the GDP-bound form of Arf1.

(Gujar et al., 2023)
FBrf0257549-3.PLA.FM
Reference
Gujar et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Arf1 

Arf1

neutral component

FLAG tag

Arf1 

Arf1

neutral component

FLAG tag

Arf1 
Arf1 

Arf1

neutral component

FLAG tag

Arf1 
Patronin 

Patronin

neutral component

Myc tag

Patronin 
Patronin 

Patronin

neutral component

Myc tag

Patronin 

Patronin

neutral component

Myc tag

Patronin 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arf1 
interaction domain residue 71
necessary binding region
aa 71

Q71L; coordinates relative to Arf1-PA

Patronin 
CKK domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from tagged, transfected construct.

(Gujar et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)