FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000122843
Interaction Network
Interactions Browser links
Plp network
poe network
Reported Interactions
FBrf0257099-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Plp 

Plp

bait

fused to GAL4 DNA-binding domain

Plp 
poe 

Pie

prey

fused to GAL4 activation domain

poe 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Plp 
interaction domain
sufficient binding region
aa 584-1811aa 2539-2895
poe 
interaction domain
sufficient binding region
aa 1-1100aa 1741-1920

coordinates relative to Poe-PA

contains conserved UBR box; coordinates relative to Poe-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0257099-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Plp 

Plp

bait

GFP tag

Plp 
poe 

Pie

prey

FLAG tag

poe 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Plp 
interaction domain
sufficient binding region
aa 1-1811
poe 
interaction domain
sufficient binding region
aa 1-1100aa 1101-2300aa 3501-5322

coordinates relative to Poe-PA

coordinates relative to Poe-PA

coordinates relative to Poe-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 0 )
References (1)