FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sumo, spn-E
FlyBase ID
FBig0000123067
Interaction Network
Interactions Browser links
Sumo network
spn-E network
Reported Interactions
FBrf0257145-12.coIP.WB
Reference
Ninova et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
spn-E 

Spn-E

bait

GFP tag

Sumo 

Sumo

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ninova et al., 2023)
FBrf0257145-4.PD.coIP.MS
Reference
Ninova et al., 2023
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

Sumo

bait

His-diGly tag

spn-E 

Spn-E

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovarian nurse cells of transgenic fly line; bait and prey produced from endogenous gene.

(Ninova et al., 2023)
FBrf0257145-8.coIP.WB
Reference
Ninova et al., 2023
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
spn-E 

Spn-E

bait

GFP tag

Sumo 

Sumo

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Knockdown of piwi reduces spn-E-Sumo interaction.

(Ninova et al., 2023)

Source was ovarian nurse cells of transgenic fly line; bait and prey produced from tagged transgenic constructs.

(Ninova et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)